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Research
Projects
Analytical
Chemistry
 The
focus of my analytical chemistry project was to identify
possible metals that have the potential to degrade
Trichloroethylene in-situ. Trichloroethylene is a chlorinated
aliphatic hydrocarbon which solubilize heavy old and greases.
Over, time large use of this solvent has lead to widespread
environmental contamination. The focus of my research was to
identify possible metals that will decrease the time for the
breakdown of this chemical without having to remove it from
the soil.
Procedures Used
in these experiments include basic molecular Techniques:
Atomic
Absorption
UV
Spectrometry
IR.
Spectrometry
Presentation
Include:
1.
A
Kinetic Study Of The Degradation Of Trichloroethylene By
Metal Ions (1997)
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Bioinformatics
 The
focus of my bioinformatics project was to identify
possible metal binding proteins in bacteria such as
Enterococcus faecalis, Staphylococcus aureus and
E.coli. These proteins may play a significant role
in a possible secondary mechanism for the destruction of these
bacteria when microwaved. Proteins that can bind metals
such as iron can be damaged when a bacteria is heated using
dielectric heating.
Procedures Used
in these experiments include :
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Microbiology
 The
focus of my microbiology project was to identify transitional
metals that may act as a sensitizer for bacteria, such as E.
faecalis, S. aureus, and E. coli, when
exposed to microwave radiation. The unique properties of
microwave radiation have been know for the past 50 years.
Currently, microwave radiation is primarily used for heating
food items. My research focused on the identification of a
non-toxic transitional metal that will allow microwave
radiation to rapidly deactivate bacteria such as E.
faecalis, S. aureus, and
E.
coli
Procedures used
in these experiments include basic molecular Techniques:
Serial
Dilution
Presentation
Include:
2. A
Novel Method For The Deactivation of Enterococcus faecalis,
Staphylococcus aureus, and Escherichia coli (2004)
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Molecular Biology
 The
focus of my molecular biology project was to identify possible
mechanisms for the bone gla protein Osteocalcin. Osteocalcin
is a protein primarily produced by the osteoblast, the bone
forming cells of the bone, its primary function is to bind
calcium in the form of hydroxyapatite to bone. My research
attempted to identify secondary mechanism for this protein by
ascertaining changes in its transcription and expression in
various body tissues such as kidney and muscle. Additionally,
other experiments examined the expression of this protein in
cartilaginous fish.
Molecular
techniques used in this experiment include :
Reverse
Transcription
Polymerase
Chain Reaction
Gel
Electrophoresis
Presentation
Include:
3.
Semi-Quantitative
Analysis Of Rat Osteocalcin mRNA Expression Between
Skeletal And Non Skeletal Cells As A Function Of
Immobilization Stress. (1995)
4.
Probing Osteocalcin
in Fish
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Toxicology
 The
focus of my toxicology project was to identify the
pharmacological effect of Silymarin has on hepatotoxicity
(liver). Silymarin is a combination of compound extracted from
the commonly found milk thistle plant. This plant has been
used for centuries to help with individuals with liver
conditions. My focus was to document Silymrian's pharmalogical
effects test animals were exposed to common hepatotoxins such
as Alpha-Naphthylisothiocyanate (ANIT) and Endotoxin.
Procedures Used
in these experiments include basic molecular Techniques:
Presentation
Include:
5.
Post
Treatment With Silymarin Reduces The Degree Of Alpha-Naphthylisothiocyanate-Induced
Liver Injury. (2002)
6.
Pretreatment
With Silymarin Modifies The Level Of Endotoxin-Induced
Neutrophil Migration And Liver Injury (2002)
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